Promoter Sequence of Shiga Toxin 2 (Stx2) Is Recognized In Vivo , Leading to Production of Biologically Active Stx2
نویسندگان
چکیده
منابع مشابه
Promoter Sequence of Shiga Toxin 2 (Stx2) Is Recognized In Vivo, Leading to Production of Biologically Active Stx2
UNLABELLED Shiga toxins (Stx) are the main agent responsible for the development of hemolytic-uremic syndrome (HUS), the most severe and life-threatening systemic complication of infection with enterohemorrhagic Escherichia coli (EHEC) strains. We previously described Stx2 expression by eukaryotic cells after they were transfected in vitro with the stx2 gene cloned into a prokaryotic plasmid (p...
متن کاملExtraintestinal pathogenic Escherichia coli carrying the Shiga Toxin gene stx2.
The 2011 outbreak of infections with Escherichia coli with characteristics of both enteroaggregative E. coli (EAEC) and Shiga toxin-producing E. coli (STEC) caused a paradigm shift with regard to the human pathogenicity of STEC (1). Diarrheagenic E. coli (DEC) usually does not cause extraintestinal diseases such as urinary tract infections and bacteremia (2). The DNA of 193 E. coli isolates fro...
متن کاملNatural killer T (NKT) cells accelerate Shiga toxin type 2 (Stx2) pathology in mice
Shiga toxin-producing Escherichia coli (STEC) is a leading cause of childhood renal disease Hemolytic Uremic Syndrome (HUS). The involvement of renal cytokines and chemokines is suspected to play a critical role in disease progression. In current article, we tested the hypothesis that NKT cells are involved in Stx2-induced pathology in vivo. To address this hypothesis we compared Stx2 toxicity ...
متن کاملStructure of shiga toxin type 2 (Stx2) from Escherichia coli O157:H7.
Several serotypes of Escherichia coli produce protein toxins closely related to Shiga toxin (Stx) from Shigella dysenteriae serotype 1. These Stx-producing E. coli cause outbreaks of hemorrhagic colitis and hemolytic uremic syndrome in humans, with the latter being more likely if the E. coli produce Stx2 than if they only produce Stx1. To investigate the differences among the Stxs, which are al...
متن کاملShiga toxin Stx2 is heat-stable and not inactivated by pasteurization.
Shiga toxin-producing Escherichia coli have been associated with food-borne illnesses. Pasteurization is used to inhibit microbial growth in milk, and an open question is whether milk pasteurization inactivates Shiga toxins. To answer this question we measured Shiga toxin's inhibition effect on Vero cell dehydrogenase activity and protein synthesis. Our data demonstrate that Shiga toxin 2 (Stx2...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: mBio
سال: 2013
ISSN: 2161-2129,2150-7511
DOI: 10.1128/mbio.00501-13